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KMID : 0861020180330040077
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Korea Journal of Herbology
2018 Volume.33 No. 4 p.77 ~ p.85
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Effects of Prunellae Spica Extract on LT4-induced Hyperthyroidism in Rats through the Regulation of Heat and Cold Imbalance
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Kang An-Na
Kang Seok-Yong
Meng Xiang-Long
Ma Jun-Nan
Park Jong-Hun
Park Yong-Ki
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Abstract
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Objective : This study was intended to examine the effects of water extract of Prunellae Spica (PS), which is a herb with 'cold' nature based on hot and cold theory of traditional Korean medicine.
Methods : Hyperthyroidism was induced in SD rats by LT4 (0.5 ㎎/㎏, i.p.) daily for four weeks. After 2 weeks of LT4 injection, rats were divided randomly into four groups; normal, LT4-induced hyperthyroid control, PS extract (500 ㎎/㎏, p.o.)-treated group, and propylthiouracil (PTU, 10 ㎎/㎏, s.c.)-treated positive group. After 2 weeks of drug treatment, all rats were sacrificed and harvested blood samples and thyroid tissues. The changes of body weight, food and water intake, and body temperature were measured weekly. Serological markers were analyzed in sera using an enzyme-based assay, and thyroid tissues were stained with Hematoxylin & Eosin (H&E). Brain and dorsal root ganglion (DRG) tissues were isolated and analyzed the expression of transient receptor potential (TRP) channels by Western blot.
Results : PS extract administration attenuated the loss of body weight and the increase of body temperature in LT4- induced hyperthyroidism rats. PS extract increased the level of thyroid stimulating hormone (TSH) and decreased tiiodothyronine (T3) and tetraiodothyronine (T4). In action mechanism, PS extract regulated the expression of transient receptor potential channel subfamily V member 1 (TRPV1) and transient Receptor Potential channel subfamily M member 8 (TRPM8), the thermoregulators.
Conclusion : To conclude, PS extract can improve the symptoms of hyperthyroidism through regulation of the thyroid hormones imbalance and thermoregulation via TRP channels.
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KEYWORD
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Prunellae Spica, LT4, Hyperthyroidism, Thyroid hormones, Thermoregulation, TRPV1, TRPM8
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